Analysis of cryoresistance of cumulus of Ovis Arias oocytes after intra- or extra-ovarian vitrification

The aim of this study was to identify the effects highly dispersed silica nanoparticles (HDSns) on cryoresistance Ovis Aries cumulus oocytes in conditions intra-(IOV) or extra-(EOV) ovarian vitrification. Materials and methods. Cumulus-oocyte complexes (COC) intended for EOV were treated with solutions cryoprotectants (CPA) prepared TC-199 10% FBS (fetal bovine serum): CPA-1-0.7 M dimethyl sulfoxide (DMSO) + 0.9 ethylene glycol (EG);CPA-2-1.4 DMSO 1.8 EG; CPA -3- 2.8 3.6 EG 0.65 trehalose. COC gradually exposed 30 seconds CPA-1, CPA-2, 20 CPA-3. At IOV, ovaries animals divided into fragments placed sterile gauze containers CPAs basis Dulbecco (PBS) 20% FBS: CPA-1 - 7.5% (25 min), then CPA-2 15% EG+15% 0.5 sucrose (15 min). 0.001% HDSns (A.A. Chuiko ICP National Academy Sciences Ukraine) added media thawing experimental groups. straws biomaterial stored liquid nitrogen at least 24 hours. COCs removed from after successively 3 min: a 0.25 solution trehalose 37°C, 0.19 M, finally 0.125 thawed sequentially (1 min) (5 PBS FBS. final washing all carried out Results. results morphological (the character oocyte-cumulus communications) cytological monitoring status cells chromatin) revealed positive effect parameters both IOV. proportion compact group gametes significantly exceeded that their absence (69 % vs. 51 %, P<0.05). A similar trend shown IOV (35 level apoptotic (TUNEL-test) (44 32 P<0.05) (72 P<0.001) sharply decreased when cryoprotective media, pyknotic during (from 31 21 58 41 In general, obtained indicate expediency using 0.001 protocols female OVIS ARIAS.